U.S. Pig Genome Coordination Program Distributed
Differential Display Primers

Updated: December, 1997

Differential Display PCR (dd-PCR) is a powerful method for elucidating differences and/or changes in gene expression between animals with divergent phenotypes. This primer set consists of 10 oligo-dT anchor antisense primers with different 2-base extensions on the 3' end, and 20 arbitrary 10-mer sense primers (5'), yielding a total of 200 primer combinations for screening cDNA populations. Each anchor primer has T7 sequence on the 5' end, while each arbitrary primer has M13 sequence on the 5' end. These extensions allow for simplified reamplification, sequencing, and expression studies of bands of interest. The set was put together by Daniel Pomp and is available for distribution. Given the more limited production of these primers, priority for distribution will be NC-210 members and pig gene researchers.

To request primers, please read the Disclaimer and Terms of Use of Primers , contact the Pig Genome Coordinator at mfrothsc@iastate.edu with your signed Disclaimer and your request. 

    

  1. acg act cac tat agg gct ttt ttt ttt ttt t   (Oligo dT)

  2. acg act cac tat agg gct ttt ttt ttt ttg a   (Anchor 1)

  3. acg act cac tat agg gct ttt ttt ttt ttg c   (Anchor 2)

  4. acg act cac tat agg gct ttt ttt ttt ttg g   (Anchor 3)

  5. acg act cac tat agg gct ttt ttt ttt ttg t   (Anchor 4)

  6. acg act cac tat agg gct ttt ttt ttt ttc a   (Anchor 5)

  7. acg act cac tat agg gct ttt ttt ttt ttc c   (Anchor 6)

  8. acg act cac tat agg gct ttt ttt ttt ttc g   (Anchor 7)

  9. acg act cac tat agg gct ttt ttt ttt tta a   (Anchor 8)

  10. acg act cac tat agg gct ttt ttt ttt tta c   (Anchor 9)

  11. acg act cac tat agg gct ttt ttt ttt tta g   (Anchor 10)

  12. aca att tca cac agg acg act cca ag          (Arbitrary 1)

  13. aca att tca cac agg agc tag cat gg          (Arbitrary 2)

  14. aca att tca cac agg aga cca ttg ca          (Arbitrary 3)

  15. aca att tca cac agg agc tag cag ac          (Arbitrary 4)

  16. aca att tca cac agg aat ggt cgt ct          (Arbitrary 5)

  17. aca att tca cac agg ata caa cga gg          (Arbitrary 6)

  18. aca att tca cac agg atg gat tgg tc          (Arbitrary 7)

  19. aca att tca cac agg atg gta aag gg          (Arbitrary 8)

  20. aca att tca cac agg ata agc cta gc          (Arbitrary 9)

  21. aca att tca cac agg aga tct cag ac          (Arbitrary 10)

  22. aca att tca cac agg aac gct agt gt          (Arbitrary 11)

  23. aca att tca cac agg agg tac taa gg          (Arbitrary 12)

  24. aca att tca cac agg agt tgc acc at          (Arbitrary 13)

  25. aca att tca cac agg atc cat gac tc          (Arbitrary 14)

  26. aca att tca cac agg act ttc tac cc          (Arbitrary 15)

  27. aca att tca cac agg atc ggt cat ag          (Arbitrary 16)

  28. aca att tca cac agg act gct agg ta          (Arbitrary 17)

  29. aca att tca cac agg atg atg cta cc          (Arbitrary 18)

  30. aca att tca cac agg att ttg gct cc          (Arbitrary 19)

  31. aca att tca cac agg atc gat aca gg          (Arbitrary 20)

  32. agc gga taa caa ttt cac aca gga             (M13 reverse (-48) primer)

  33. gta ata cga ctc act ata ggg c               (T7 promoter)

 

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