Set IX Fluorescent Primer Information

Table 10
Set XI (diversity) Fluorescent Primer Information
(Made in October 2001)

Symbol Chromosome Fluorescent Dye Type Forward Primer Length Reverse Primer Length

CGA 1 HEX GENE ATAGACATTATGTAAGTTGCTGAT 24 GAACTTTCACATCCCTAAGGTCGT 24

S0155 1 FAM DNA TGTTCTCTGTTTCTCCTCTGTTTG 24 AAAGTGGAAAGAGTCAATGGCTAT 24

SW1828 1 TET DNA AATGCATTGTCTTCATTCAACC 22 TTAACCGGGGCACTTGTG 18

S0226 2 FAM DNA GGTTAAACTTTTNCCCCAATACA 23 GCACTTTTAACTTTCATGATGCTCC 25

SW240 2 TET DNA AGAAATTAGTGCCTCAAATTGG 22 AAACCATTAAGTCCCTAGCAAA 22

S0002 3 HEX DNA GAAGCCAAAGAGACAACTGC 20 GTTCTTTACCCACTGAGCCA 20

SW72 3 FAM DNA ATCAGAACAGTGCGCCGT 18 TTTGAAAATGGGGTGTTTCC 20

SW902 3 FAM DNA ATCAGTTGGAAATGATGGCC 20 CTTGCCTCAAAGAGTTGTAAGG 22

S0097 4 TET DNA GACCTATCTAATGTCATTATAGT 23 TTCCTCCTAGAGTTGACAAACTT 23

S0217 4 TET DNA TGTGATGCAGGCTGGCAG 18 GCCTCCTCATCTGGGGTC 18

S0227 4 HEX DNA GATCCATTTATAATTTTAGCACAAAGT 27 GCATGGTGTGATGCTATGTCAAGC 24

S0301 4 FAM DNA CCGTCTTACTTAGGATGTTT 20 TGATGTGTTTATGTGTTTGA 20

IGF1 5 FAM GENE ACCCTTGAGAGGGTATTGCTAGGC 24 GGGCAAAGATTCTGCTGAGCTG 22

S0005 5 TET DNA TCTTCCCTCCTGGTAACTA 20 GCACTTCCTGATTCTGGGTA 20

S0228 6 TET DNA GGCATAGGCTGGCAGCAACA 20 AGCCCACCTCATCTTATCTACACT 24

SW1067 6 HEX DNA TGCTGGCCAGTGACTCTG 18 CCGGGGGATTAAACAAAAAG 20

SW122 6 FAM DNA TTGTCTTTTTATTTTGCTTTTGG 23 CAAAAAAGGCAAAAGATTGACA 22

SW2406 6 TET DNA AATGTCACCTTTAAGACGTGGG 22 AATGCGAAACTCCTGAATTAGC 22

S0025 7 FAM DNA TCTCCCTTCCCTCCATCTCT 20 CTCCATCAGCCAAAAACATT 20

S0101 7 HEX DNA GAATGCAAAGAGTTCAGTGTAGG 23 GTCTCCCTCACACTTACCGCAG 22

SW632 7 TET DNA TGGGTTGAAAGATTTCCCAA 20 GGAGTCAGTACTTTGGCTTGA 21

S0178 8 TET DNA TAGCCTGGGAACCTCCACACGCTG 24 GGCACCAGGAATCTGCAATCCAGT 24

S0225 8 HEX DNA GCTAATGCCAGAGAAATGCAGA 22 CAGGTGGAAAGAATGGAATGAA 22

SW2410 8 HEX DNA ATTTGCCCCCAAGGTATTTC 20 CAGGGTGTGGAGGGTAGAAG 20

SW174 9 TET DNA GCCAAAATAGCTATTGGACAGC 22 TCATGCTATTTTGTTCCAGATG 22

SW911 9 FAM DNA CTCAGTTCTTTGGGACTGAACC 22 CATCTGTGGAAAAAAAAAGCC 21

S0070 10 HEX DNA GGCGAGCATTTCATTCACAG 20 GAGCAAACAGCATCGTGAGC 20

SW830 10 FAM DNA AAGTACCATGGAGAGGGAAATG 22 ACATGGTTCCAAAGACCTGTG 21

SW951 10 HEX DNA TTTCACAACTCTGGCACCAG 20 GATCGTGCCCAAATGGAC 18

S0386 11 TET DNA TCCTGGGTCTTATTTTCTA 19 TTTTTATCTCCAACAGTAT 19

SW2008 11 HEX DNA CAGGCCAGAGTAGCGTGC 18 CAGTCCTCCCAAAAATAACATG 22

S0090 12 FAM DNA CCAAGACTGCCTTGTAGGTGAATA 24 GCTATCAAGTATTGTACCATTAGG 24

S0143 12 TET DNA ACTCACAGCTTGTCCTGGGTGT 22 CAGTCAGCAGGCTGACAAAAAC 22

S0068 13 TET DNA AGTGGTCTCTCTCCCTCTTGCT 22 CCTTCAACCTTTGAGCAAGAAC 22

S0215 13 HEX DNA TAGGCTCAGACCCTGCTGCAT 21 TGGGAGGCTGAAGGATTGGGT 21

SW769 13 FAM DNA GGTATGACCAAAAGTCCTGGG 21 TCTGCTATGTGGGAAGAATGC 21

SWR1941 13 TET DNA AGAAAGCAATTTGATTTGCATAATC 25 ACAAGGACCTACTGTATAGCACAGG 25

SW295 14 TET DNA ACCTGCCAGAGTTGTGGC 18 AAGAGTTTCATTTCTCCCATCC 22

SW857 14 HEX DNA TGAGAGGTCAGTTACAGAAGACC 23 GATCCTCCTCCAAATCCCAT 20

S0355 15 FAM DNA TCTGGCTCCTACACTCCTTCTTGATG 26 TTGGGTGGGTGCTGAAAAATAGGA 24

SW1111 15 TET DNA AGGTCCTACTGTCCATCACAGG 22 GAAGCAGAGTTGGCTTACAGTG 22

SW936 15 FAM DNA TCTGGAGCTAGCATAAGTGCC 21 GTGCAAGTACACATGCAGGG 20

S0026 16 HEX DNA AACCTTCCCTTCCCAATCAC 20 CACAGACTGCTTTTTACTCC 20

SW742 16 HEX DNA AATTCTACTTCTGGGGAGAGGG 22 CTTTTGGGAACATTTCTGCC 20

SW24 17 TET DNA CTTTGGGTGGAGTGTGTGC 19 ATCCAAATGCTGCAAGCG 18

SWR1004 17 FAM DNA TGGGAACACCTGCTTCATTC 20 TCCATATGCCCCAAGTGTG 19

SW1023 18 FAM DNA AACCTGCTGAGCCACAGTG 19 GCAAGTACCCAATCTTTTTTCC 22

SW787 18 FAM DNA CTGGAGCAGGAGAAAGTAAGTTC 23 GGACAGTTACAGACAGAAGAAGG 23

S0218 X TET DNA GTGTAGGCTGGCGGTTGT 18 CCCTGAAACCTAAAGCAAAG 20

SW2476 X HEX DNA GAGAGGGACAGAGCTGAGAGC 21 CTTGAGGTTTGATGGCACG 19

The availability of older sets is limited. To request fluorescent primers, please either:

Use the online form to fill out your request (recommend),
or

Contact the U.S. Pig Genome Coordinator Max Rothschild at the address given below, and be sure to provide the following: (1) which primer set you request; (2) your complete postal address (NOT a P.O.Box); (3) your day-time phone number and (4) a signed copy of the Disclaimer and Terms of Use of Primers.

            Max F. Rothschild
            2255 Kildee Hall
            Department of Animal Science
            Iowa State University
            Ames, Iowa 50011
            Phone: 515-294-6202
            Fax:   515-294-2401
            Email: mfrothsc@iastate.edu

Symbol	Chromosome	Fluorescent Dye	Type	Forward Primer	Length	Reverse Primer	Length
CGA	1	HEX	GENE	ATAGACATTATGTAAGTTGCTGAT	24	GAACTTTCACATCCCTAAGGTCGT	24
S0155	1	FAM	DNA	TGTTCTCTGTTTCTCCTCTGTTTG	24	AAAGTGGAAAGAGTCAATGGCTAT	24
SW1828	1	TET	DNA	AATGCATTGTCTTCATTCAACC	22	TTAACCGGGGCACTTGTG	18
S0226	2	FAM	DNA	GGTTAAACTTTTNCCCCAATACA	23	GCACTTTTAACTTTCATGATGCTCC	25
SW240	2	TET	DNA	AGAAATTAGTGCCTCAAATTGG	22	AAACCATTAAGTCCCTAGCAAA	22
S0002	3	HEX	DNA	GAAGCCAAAGAGACAACTGC	20	GTTCTTTACCCACTGAGCCA	20
SW72	3	FAM	DNA	ATCAGAACAGTGCGCCGT	18	TTTGAAAATGGGGTGTTTCC	20
SW902	3	FAM	DNA	ATCAGTTGGAAATGATGGCC	20	CTTGCCTCAAAGAGTTGTAAGG	22
S0097	4	TET	DNA	GACCTATCTAATGTCATTATAGT	23	TTCCTCCTAGAGTTGACAAACTT	23
S0217	4	TET	DNA	TGTGATGCAGGCTGGCAG	18	GCCTCCTCATCTGGGGTC	18
S0227	4	HEX	DNA	GATCCATTTATAATTTTAGCACAAAGT	27	GCATGGTGTGATGCTATGTCAAGC	24
S0301	4	FAM	DNA	CCGTCTTACTTAGGATGTTT	20	TGATGTGTTTATGTGTTTGA	20
IGF1	5	FAM	GENE	ACCCTTGAGAGGGTATTGCTAGGC	24	GGGCAAAGATTCTGCTGAGCTG	22
S0005	5	TET	DNA	TCTTCCCTCCTGGTAACTA	20	GCACTTCCTGATTCTGGGTA	20
S0228	6	TET	DNA	GGCATAGGCTGGCAGCAACA	20	AGCCCACCTCATCTTATCTACACT	24
SW1067	6	HEX	DNA	TGCTGGCCAGTGACTCTG	18	CCGGGGGATTAAACAAAAAG	20
SW122	6	FAM	DNA	TTGTCTTTTTATTTTGCTTTTGG	23	CAAAAAAGGCAAAAGATTGACA	22
SW2406	6	TET	DNA	AATGTCACCTTTAAGACGTGGG	22	AATGCGAAACTCCTGAATTAGC	22
S0025	7	FAM	DNA	TCTCCCTTCCCTCCATCTCT	20	CTCCATCAGCCAAAAACATT	20
S0101	7	HEX	DNA	GAATGCAAAGAGTTCAGTGTAGG	23	GTCTCCCTCACACTTACCGCAG	22
SW632	7	TET	DNA	TGGGTTGAAAGATTTCCCAA	20	GGAGTCAGTACTTTGGCTTGA	21
S0178	8	TET	DNA	TAGCCTGGGAACCTCCACACGCTG	24	GGCACCAGGAATCTGCAATCCAGT	24
S0225	8	HEX	DNA	GCTAATGCCAGAGAAATGCAGA	22	CAGGTGGAAAGAATGGAATGAA	22
SW2410	8	HEX	DNA	ATTTGCCCCCAAGGTATTTC	20	CAGGGTGTGGAGGGTAGAAG	20
SW174	9	TET	DNA	GCCAAAATAGCTATTGGACAGC	22	TCATGCTATTTTGTTCCAGATG	22
SW911	9	FAM	DNA	CTCAGTTCTTTGGGACTGAACC	22	CATCTGTGGAAAAAAAAAGCC	21
S0070	10	HEX	DNA	GGCGAGCATTTCATTCACAG	20	GAGCAAACAGCATCGTGAGC	20
SW830	10	FAM	DNA	AAGTACCATGGAGAGGGAAATG	22	ACATGGTTCCAAAGACCTGTG	21
SW951	10	HEX	DNA	TTTCACAACTCTGGCACCAG	20	GATCGTGCCCAAATGGAC	18
S0386	11	TET	DNA	TCCTGGGTCTTATTTTCTA	19	TTTTTATCTCCAACAGTAT	19
SW2008	11	HEX	DNA	CAGGCCAGAGTAGCGTGC	18	CAGTCCTCCCAAAAATAACATG	22
S0090	12	FAM	DNA	CCAAGACTGCCTTGTAGGTGAATA	24	GCTATCAAGTATTGTACCATTAGG	24
S0143	12	TET	DNA	ACTCACAGCTTGTCCTGGGTGT	22	CAGTCAGCAGGCTGACAAAAAC	22
S0068	13	TET	DNA	AGTGGTCTCTCTCCCTCTTGCT	22	CCTTCAACCTTTGAGCAAGAAC	22
S0215	13	HEX	DNA	TAGGCTCAGACCCTGCTGCAT	21	TGGGAGGCTGAAGGATTGGGT	21
SW769	13	FAM	DNA	GGTATGACCAAAAGTCCTGGG	21	TCTGCTATGTGGGAAGAATGC	21
SWR1941	13	TET	DNA	AGAAAGCAATTTGATTTGCATAATC	25	ACAAGGACCTACTGTATAGCACAGG	25
SW295	14	TET	DNA	ACCTGCCAGAGTTGTGGC	18	AAGAGTTTCATTTCTCCCATCC	22
SW857	14	HEX	DNA	TGAGAGGTCAGTTACAGAAGACC	23	GATCCTCCTCCAAATCCCAT	20
S0355	15	FAM	DNA	TCTGGCTCCTACACTCCTTCTTGATG	26	TTGGGTGGGTGCTGAAAAATAGGA	24
SW1111	15	TET	DNA	AGGTCCTACTGTCCATCACAGG	22	GAAGCAGAGTTGGCTTACAGTG	22
SW936	15	FAM	DNA	TCTGGAGCTAGCATAAGTGCC	21	GTGCAAGTACACATGCAGGG	20
S0026	16	HEX	DNA	AACCTTCCCTTCCCAATCAC	20	CACAGACTGCTTTTTACTCC	20
SW742	16	HEX	DNA	AATTCTACTTCTGGGGAGAGGG	22	CTTTTGGGAACATTTCTGCC	20
SW24	17	TET	DNA	CTTTGGGTGGAGTGTGTGC	19	ATCCAAATGCTGCAAGCG	18
SWR1004	17	FAM	DNA	TGGGAACACCTGCTTCATTC	20	TCCATATGCCCCAAGTGTG	19
SW1023	18	FAM	DNA	AACCTGCTGAGCCACAGTG	19	GCAAGTACCCAATCTTTTTTCC	22
SW787	18	FAM	DNA	CTGGAGCAGGAGAAAGTAAGTTC	23	GGACAGTTACAGACAGAAGAAGG	23
S0218	X	TET	DNA	GTGTAGGCTGGCGGTTGT	18	CCCTGAAACCTAAAGCAAAG	20
SW2476	X	HEX	DNA	GAGAGGGACAGAGCTGAGAGC	21	CTTGAGGTTTGATGGCACG	19