U.S. Pig Genome Coordination Program
Distributed Fluorescent Primers
Graphic view of markers on each chromosome is avaliable now
Updated on April 30, 2000
NOTE | 1. | Primers are ordered on the chromosomal map starting from the tip of short arm (p) to the tip of long (q) arm within a chromosome. Clicking on the marker name will bring you more information about the marker. |
2. | Relative location values (cM) were retrieved from the USDA-MARC Swine Genome Map except those markers whose names were labeled with *. The relative location (cM) of markers labeled with * were obtained from PIGBASE. | |
3. | For each primer pair in Set III, Set IV, Set V, Set VI, Set VII and Set VIII, only the forward primer is labeled with fluorescent dye. Fluorescent labeled primer information for Sets I/II are indicated along with detailed primer information (click on marker name). | |
4. | The suggested multiplex groups are based on information kindly provided by Rohrer et al. "Mapping 28 erythrocyte antigen, plasma protein and enzyme polymorphisms using an efficient genomic scan of the porcine genome" (Animal Genetics, 28:323-330). | |
5. | All primers were made and therefore available in groups. Clicking on the group name will bring you the primer set information. | |
6. | Operon recommendation for storage: It is recommended that the oligos be brought up in a slightly basic solution (i.e. pH=8). If they are brought up at pH<7 (deionized water is pH=5), it has been shown that the oligo can begin to degrade as soon as within a couple of weeks. |
The availability of older sets is limited. To request fluorescent primers, please either:
or
Max F. Rothschild 2255 Kildee Hall Department of Animal Science Iowa State University Ames, Iowa 50011 Phone: 515-294-6202 Fax: 515-294-2401 Email: mfrothsc@iastate.edu
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USA · USDA · NRPSP8 · Program to Accelerate Animal Genomics Applications.
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