Primers from U.S. Pig Genome Coordination Project

U.S. Pig Genome Coordination Program
Distributed Fluorescent Primers

Updated on October 28, 2001

A new set of 50 pig microsatellite diversity primer pairs is available. This set of primers was made in response to requests and suggestions concerning pig diversity research and selected from markers across all 19 pig chromosomes. These were suggested by several people and should work nicely to study diversity in different breeds. Visit Table 11 for further information on this primer set. The total number of fluorescent primer pairs distributed by the U.S. Pig Genome Coordinator is now 559 pairs (Table 1; All markers are ordered by chromosomal locations) which includes nine fluorescent primer sets:

Sets Size List Produced Availibility

Set I 63 primer pairs Table 2 Feburary, 1996 0

Set II 33 primer pairs Table 2 September, 1996 0

Set III 52 primer pairs Table 3 August, 1997
September 2001 0

Set IV 53 primer pairs Table 4 March, 1998 0

Set V 24 primer pairs Table 5 May, 1998 0

Set VI 28 primer pairs Table 6 September, 1998 0

Set VII 51 primer pairs Table 7 December, 1998 0

Set VIII 73 primer pairs Table 8 May, 1999 0

Set IX 91 primer pairs Table 9 January, 2000 0

Set X 41 primer pairs Table 10 April, 2001 0

Set XI (diversity) 50 primer pairs Table 11 October, 2001 0

Criteria used for the marker selection and priorities given for the marker selected:

Even coverage of the entire length of the porcine genome; (So far we have at least one marker every 5-10cM on each chromosome).
Those physically anchored ones;
Those closest to and around interesting known coding genes/QTLs.
Those recommended/ requested by the swine gene mapping coordination project participants;
The PCR product sizes and fluorescent dyes have been chosen in such a way so that multiplexing is possible.

The availability of older sets is limited. To request fluorescent primers, please either:

Use the online form to fill out your request (recommend),
or

Contact the U.S. Pig Genome Coordinator Max Rothschild at the address given below, and be sure to provide the following: (1) which primer set you request; (2) your complete postal address (NOT a P.O.Box); (3) your day-time phone number and (4) a signed copy of the Disclaimer and Terms of Use of Primers.
To: Max F. Rothschild 2255 Kildee Hall Department of Animal Science Iowa State University Ames, Iowa 50011 Phone: 515-294-6202 Fax: 515-294-2401 Email: mfrothsc@iastate.edu